Product Information

VitroFlo can be used in several mono and co-culture configurations. Applications include modeling barrier function, biochemical signalling, drug absorption, and more. With no pumps or tubing and the ability to run 12 independent tests in a single wellplate, the VitroFlo is sure to accelerate you to your research goals.

2D Flow Wellplates

The 2D wellplate provides continuous fluid shear over a cell monolayer. The rocker plate controls flow rates to generate mild perfusion to high shear conditions. A glass cover layer allows for clear fluorescent and confocal microscopy. 

channel area49 mm2
channel height286 µm
cover layer (culture surface)glass
wellplate materialpolystyrene

2D/2D Co-Culture Wellplates

This version of the co-culture support 2 different cell monolayers on either side of a porous membrane. Controlled flow occurs in the upper channel and imaging can be done in both channels.

channel area (Upper)49 mm2
channel height (Upper)286 µm
channel area (Lower)125 mm2
channel height (Lower)286 um
cover layer (culture surface)glass
wellplate materialpolystyrene

2D/3D Co-Culture Wellplates

In this version of the co-culture, the lower channel contains a cell-seeded hydrogel or other 3D cell matrix. Controlled flow occurs in the upper channel and imaging can be done in both channels.

channel area (Upper)49 mm2
channel height (Upper)286 µm
channel area (Lower)125 mm2
channel height (Lower)286 um
cover layer (culture surface)glass
wellplate materialpolystyrene

VitroFlo Rocker

There are 4 protocols pre-programmed onto the VitroFlo Rocker. These offer a range of flow rates for cell stimulations.

Position 1: perfusion flow, 1dynes/cm2 shear, 200µL/min flow

Position 2: mild shear, 3dynes/cm2 shear, 600µL/min flow

Position 3: medium shear, 6dynes/cm2 shear, 1100µL/min flow

Position 4, high shear, 11dynes/cm2 shear, 2000µL/min flow

The Rocker can be operated inside an incubator without a PC connection.