Product Information
VitroFlo can be used in several mono and co-culture configurations. Applications include modeling barrier function, biochemical signalling, drug absorption, and more. With no pumps or tubing and the ability to run 12 independent tests in a single wellplate, the VitroFlo is sure to accelerate you to your research goals.
2D Flow Wellplates
The 2D wellplate provides continuous fluid shear over a cell monolayer. The rocker plate controls flow rates to generate mild perfusion to high shear conditions. A glass cover layer allows for clear fluorescent and confocal microscopy.

channel area | 49 mm2 |
channel height | 286 µm |
cover layer (culture surface) | glass |
wellplate material | polystyrene |
2D/2D Co-Culture Wellplates
This version of the co-culture support 2 different cell monolayers on either side of a porous membrane. Controlled flow occurs in the upper channel and imaging can be done in both channels.

channel area (Upper) | 49 mm2 |
channel height (Upper) | 286 µm |
channel area (Lower) | 125 mm2 |
channel height (Lower) | 286 um |
cover layer (culture surface) | glass |
wellplate material | polystyrene |
2D/3D Co-Culture Wellplates
In this version of the co-culture, the lower channel contains a cell-seeded hydrogel or other 3D cell matrix. Controlled flow occurs in the upper channel and imaging can be done in both channels.

channel area (Upper) | 49 mm2 |
channel height (Upper) | 286 µm |
channel area (Lower) | 125 mm2 |
channel height (Lower) | 286 um |
cover layer (culture surface) | glass |
wellplate material | polystyrene |
VitroFlo Rocker
There are 4 protocols pre-programmed onto the VitroFlo Rocker. These offer a range of flow rates for cell stimulations.
Position 1: perfusion flow, 1dynes/cm2 shear, 200µL/min flow
Position 2: mild shear, 3dynes/cm2 shear, 600µL/min flow
Position 3: medium shear, 6dynes/cm2 shear, 1100µL/min flow
Position 4, high shear, 11dynes/cm2 shear, 2000µL/min flow
The Rocker can be operated inside an incubator without a PC connection.
