We make your cell cultures more ALIVE

Our solution is a simple in vitro culture system that mimics key in vivo conditions

 

 

 

 

  

In Research

Our mission is to provide equipment for the study of cells in physiologically relevant conditions. We aim to have this equipment successfully used by a diverse group of researchers.

In Drug Development

Our mission is to impact human health by improving candidate drug selection, reducing animal testing, and increasing clinical trial success rates.

Simple

Single use components. No pumps or tubing. No special training required.

Media Circulation

Continuous unidirectional flow at controlled rates.

Customizable

2D, 3D, and co-culture cell cultures. High content imaging compatible.

The VitroFlo Approach

 

The VitroFlo system is comprised of a microfluidic cell culture plate and a rocker mechanism. Variations in the culture plate channel geometry, membrane porosity, and channel spacing support a variety of models and cell types. The programmable rocker mechanism can achieve a variety of media flow conditions and fluid shear rates.

VitroFlo Culture Plate

 

The VitroFlo patent-pending culture plate contains paired media reservoirs connected by one or more microfluidic channels to create a recirculating flow cell. The culture plates are compatible with manual and automated liquid handling and imaging systems.

Media Circulation with VitroFlo

 

Rocking the VitroFlo plate creates flow in one direction through the microfluidic cell culture channels and flow in the other direction through bypass channels. The results are controlled, near-continuous unidirectional flow and shear rates for cultured cells.  This delivers nutrients, removes waste, and stimulates cells.

Cell Co-Culture

 

The VitroFlo plate has a second microfluidic channel that is separated from the flow channel by a thin porous membrane. This channel can be used to culture a second cell type or monitor transport across the cells lining the upper channel. By combining two cell culture channels with a variety of fluidic conditions, the VitroFlo system can be used for a wide range of applications. We are proud of the work being done by our users and we are actively looking for new partners.

VitroFlo Options

2D Flow

Unidirectional laminar fluid flows through a 2mm wide microfluidic channel. Fluid shear rates of up to 11 dynes/cm2.

2D/2D Co-Culture

An upper and a lower channel separated by a thin porous membrane. Cells can be cultured on both sides of the membrane and accessed from separate ports.

2D/3D Co-Culture

The lower channel can consist of a hydrogel with a suspension of cells. Controlled flow occurs at the upper channel.

“I have been working with the team since the beginning of the VitroFlo project. The VitroFlo system is enhancing my research on endothelial barrier function and flow-induced shear responses. The current beta product is unlike any other product on the market and I am confident it will propel my research to new levels.”  

Dr. Craig Simmons

Professor, Canada Research Chair in Mechanobiology

The VitroFlo Recirculating Flow

Product Launch Presentation

A Quick Setup Guide

Get in Touch

We are eagerly seeking collaborators to test the device for their applications. If you are interested in the VitroFlo and would like more information, please fill in the form below.

 

Contact Us

 

564 Weber St N, Unit 11
Waterloo, ON, N2L 5C6
Canada

Tel: 519-342-6870
info@vitroflo.com