We make your cell cultures more ALIVE
Our solution is a simple in vitro culture system that mimics key in vivo conditions
Our mission is to provide equipment for the study of cells in physiologically relevant conditions. We aim to have this equipment successfully used by a diverse group of researchers.
In Drug Development
Our mission is to impact human health by improving candidate drug selection, reducing animal testing, and increasing clinical trial success rates.
Single use components. No pumps or tubing. No special training required.
Continuous unidirectional flow at controlled rates.
2D, 3D, and co-culture cell cultures. High content imaging compatible.
The VitroFlo Approach
The VitroFlo system is comprised of a microfluidic cell culture plate and a rocker mechanism. Variations in the culture plate channel geometry, membrane porosity, and channel spacing support a variety of models and cell types. Modifications to the rocker mechanism protocol can achieve a variety media flow conditions.
VitroFlo Culture Plate
The VitroFlo patent-pending culture plate contains paired media reservoirs connected by one or more microfluidic channels to create a recirculating flow cell. The geometry, connectivity, and pathway of these microfluidic channels can be customized to meet research needs. The culture plates are compatible with manual and automated liquid handling and imaging systems.
Media Circulation with VitroFlo
Rocking the VitroFlo culture plate creates asymmetric flow conditions. In one tilt direction, the media flows through the microfluidic cell culture channels. In the other tilt directions, the media flows through large bypass channels. The result is near-continuous unidirectional forward flow at controlled flow rates. This delivers nutrients, removes waste, and stimulates cells.
The Future of VitroFlo
Development is underway to optimize the VitroFlo for a variety of cell types. Some of the most promising applications include extending the viability of primary hepatocyte cultures, testing endothelial/organ barrier function using membrane-separated microfluidic channels, and embedding sensors into the microfluidic channels for real-time culture monitoring. We are proud of the work being done by our current beta group and we are actively looking for new partners.
“I have been working with the CellScale team since the beginning of the VitroFlo project. The VitroFlo system is enhancing my research on endothelial barrier function and flow-induced shear responses. The current beta product is unlike any other product on the market and I am confident it will propel my research to new levels.”
VitroFlo Beta Program
The Vitroflo team is currently seeking beta users. Read more about it in the brochure and please fill out the form below if you would like to participate.